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目的 检测miR-888在肝癌细胞中的表达情况,并进一步在分子水平研究其相关作用机制。方法 实时荧光定量PCR(qRT-PCR)检测肝癌细胞系中miR-888的表达情况;细胞计数试剂盒BromosporineDMSO溶解度-8(cell counting kit-8,CCK-8)法检测miR-888对Huh7细胞活性的影响;平板克隆形成实验、细胞凋亡实验、Transwell实验检测miR-888对Huh7细胞集落形成、细胞凋亡、迁移和侵袭能力的影响;荧光素酶报告检测miR-888与候选基因RB1的靶向关系,qRT-PCR和免疫印迹实验(Western blotting)检测RB1 mRNA和pRB蛋白水平的改变。